Associate Research Scientist and Lecturer
Department of Bioengineering
University of California - San Diego

M.D., Loma Linda University
Loma Linda, CA 1985

Ph.D., University of California - San Diego,
La Jolla, CA 1990

Email:

JavaScript is required to view this email address

The central theme is the development of fully automated quantitative microscopy, or scanning cytometry, for biomedical applications. The instrumentation research areas include high performance autofocus, real-time image segmentation and image fluorometry. These are key components for a scanning cytometer capable of accurate, exhaustive analyses of the 100,000 – 10,000,000 cells on a single microscope slide at optical resolution (usually ≤ 0.5 x 0.5 mm² pixels). Quantifying the measurement accuracy and/or precision of each step in the scanning cytometry process (e.g., autofocus followed by image segmentation, fluorometry and cell classification) are key components of the research. Current and proposed biomedical research areas include cervical (Pap smear) cancer screening, rare event detection (e.g., locating fetal cells in maternal circulation for genotyping), and chemotherapy testing using time-lapse scanning cytometry of living cells cultured on the microscope stage.

Original Refereed Journal Articles:

1. S Heynen, JH Price, "Optical Feedback Stabilized Mercury Vapor Arc Lamp for Image Cytometry," Review of Scientific Instruments, in Preparation.
2. S Bajaj, JB Welsh, RC Leif, JH Price, "Ultra-Rare-Event Detection Performance of a Custom Scanning Cytometer on a Model Preparation of Fetal nRBCs," Cytometry, 39:285-294, 2000.
3. M Oliva, ME Bravo-Zanoguera, JH Price, "Filtering out contrast reversals for microscopy autofocus," Applied Optics, Optical Tech. & Biomed. Optics, 38(4):638-646, 1999. Issue Cover.
4. M Bravo-Zanoguera, B von Massenbach, AL Kellner and JH Price, "High Performance Autofocus Circuit for Biological Microscopy," Review of Scientific Instruments, 69(11):3966-3977, 1998.
5. JH Price, EA Hunter, and DA Gough, "Accuracy of Least Squares Designed Spatial FIR Filters for Segmentation of Images of Fluorescence Stained Cell Nuclei," Cytometry, 25(4): 303 - 316, 1996. 1998 Annual Cover.
6. RM Schinagl, MK Ting, JH Price, RL Sah, "Video Microscopy to Quantitate the Inhomogeneous Confined Compression Modulus of Articular Cartilage," Annals of Biomedical Engineering, 24(4): 500 - 512, 1996.
7. GY Fan, B Russ, JB Talbot, JH Price and MH Ellisman, "Thin-Foil Based TEM Screens with Electrophoretic Deposition of Phosphors," Journal of the Microscopy Society of America Proceedings, pp. 30-31, August 13-17, 1995.
8. JH Price DA Gough, "Comparison of Digital Autofocus Functions for Phase-Contrast and Fluorescence Scanning Microscopy," Cytometry, 16(4): 283 - 297, 1994.

Proceedings and Abstracts

1. S Heynen, EA Hunter, JH Price, "Cell-by-cell classification strategies for scanning cytometry," International Society for Analytical Cytology (ISAC) XX International Congress, Montpellier, France, May 2000.
2. LK Nguyen, M Bravo-Zanoguera, AL Kellner, JH Price, "Magnification Corrected Optical Image Splitting Module for Simultaneous Multiplanar Acquisition," Proc. Of SPIE, Optical Diagnostics of Living Cells III, 3921:31-40, 2000.
3. EL Botvinick, S Cha, FF Li, DA Gough, Y Fainman, JH Price, "In vivo confocal microscopy based on the Texas Instruments Digital Micromirror Device," Proc. Of SPIE, Optical Diagnostics of Living Cells III, San Jose, 3921: 12-19, 2000.
4. S Heynen, JH Price, "Review of Cell Nuclei Features for Classification from Fluorescence Images," Proc. Of SPIE, Optical Diagnostics of Living Cells III, 3921:54-65, 2000.
5. EA Hunter, WS Callaway, JH Price, "Software Framework for Scanning Cytometry," Proc. Of SPIE, Optical Diagnostics of Living Cells III, 3921:41-52, 2000.
6. S Heynen, JH Price, "Evaluation of scanning cytometer fluorometry performance," SPIE Proc. Optical Diagnostics of Biological Fluids and Advanced Techniques Analytical Cytology, San Jose, 3604:237-242, January 1999.
7. M Bravo-Zanoguera, Bv Massenbach, JH Price, "Automatic on-the-fly focusing for continuous image acquisition in high-resolution microscopy," SPIE Proc. Optical Diagnostics of Biological Fluids and Advanced Techniques Analytical Cytology, San Jose, 3604:243-252, January 1999.
8. ME Bravo-Zanoguera, JH Price, "Simultaneous Multiplanar Image Acquisition in Light Microscopy," SPIE Proc. Optical Diagnostics of Biological Fluids and Advanced Techniques Analytical Cytology 3260: 194-200, 1998.
9. Heynen, JH Price, "Evaluation of SNAKES for an Objective Image Segmentation Standard on Cell Nuclei," Cytometry Supplement 9 (Int Soc Analyt Cytol XIX Int Congress) p. 123, 1998.
10. JH Price, "Autofocus vs. Voxel Projection for Vertical Tracking in Scanning Cytometry," SPIE Photonics West '97, vol. 2982: 485-489, Feb. 8 - 14, 1997.
11. S Heynen, DA Gough, JH Price, "Optically Stabilized Mercury Vapor Short Arc Lamp as UV-Light Source for Microscopy," SPIE Photonics West '97, vol. 2982: 430-434, Feb. 8 - 14, 1997.
12. GY Fan, T Deerinck, CC Ahn , JH Price, SJ Young and MH Ellisman. "Applications of a Virtual Objective Aperture in TEM." Proceedings of the 51st Annual Meeting of the Microscopy Society of America, pp. 634-635, August, 1993.
13. N Rowlands, JH Price, M Kersker, S Young and M Ellisman, "Design and Calibration of an IVEM for General 3D Imaging and Non-Diffracting Materials," Proceedings of the Annual Meeting of the Electron Microscopy Society, part II, pp. 1040-1041, August, 1992.
14. RM Hoffman, KM Connors, AZ Meerson-Monosov, H Herrera, and JH Price, "A General Native-State Method for Determination of Proliferation Capacity of Normal and Tumor Tissues In Vitro," Proc. Nat. Acad. Sci. U. S. A., 86:2013-17, 1989.
    
    
    
    

Dissertation

• JH Price, Scanning Cytometry for Cell Monolayers, Ph.D. Dissertation, Applied Mechanics and Engineering Sciences, Bioengineering, University of California San Diego, 1990.
  
  

Patents

• M Ellisman, G Fan, JH Price, S Suzuki, "Enhanced Imaging Mode for Transmission Electron Microscopy," US Patent 5,414,261, 6 pages, 1995.
  
  
• JH Price and DA Gough. "Operator Independent Image Cytometer," US Patent No. 5,548,661, August 20, 1996.
  
  
• JH Price and DA Gough. "Method and Means of Least Squares Designed Filters for Image Segmentation in Scanning Cytometry," US Patent No. 5,790,692, 32 pages, August 4, 1998.
  
  
• JH Price and DA Gough. "Autofocus System for Scanning Microscopy," US Patent No. 5,790,710, 35 pages, August 4, 1998.
  
  
• JH Price and DA Gough, "Arc Lamp Stabilization and Intensity Control for Imaging Microscopy," US Patent No. 5,856,665, 31 pages, January 5, 1999.